Materials

1. 0.6ml MICROTUBES:
   • Manufacturer: Genesee Scientific (CAT#: 22-272)
2. 1.7ml MICROTUBES:
   • Manufacturer: Genesee Scientific (CAT#: 24-281 & 24-282)
3. 2.0ml MICROTUBES:
   • Manufacturer: Genesee Scientific (CAT#: 22-283)
4. 5.0ml EPPENDORF TUBES:
   • Manufacturer: Eppendorf (CAT#: 0030119401)
5. 15ml CENTRIFUGE TUBES:
   • Manufacturer: Thermo Fisher Scientific (CAT#: 14-959-53A)
6. 16S FORWARD GENE PRIMER:
   • Manufacturer: Integrated DNA Technologies (CAT#: 51-01-19-06)
7. 16S REVERSE GENE PRIMER:
   • Manufacturer: Integrated DNA Technologies (CAT#: 51-01-19-07)
8. ACETIC ACID (MSDS):
   • Manufacturer: Thermo Fisher Scientific (CAT#: 64-19-7)
9. ACETONE (MSDS):
   • Manufacturer: Sigma-Aldrich (CAT#: 67-64-1)
10. ACID BLUE 29 (MSDS):
    • Manufacturer: Santa Cruz Biotechnology (CAT#: sc-214471)
11. ACID BLUE 45 (MSDS):
    • Manufacturer: Acros Organics (CAT#: 2861-02-1)
12. ACID BLUE 80 (MSDS):  
    • Manufacturer: Acros Organics (CAT#: 4474-24-2)
13. AGAROSE (MSDS):
    • Manufacturer: Thermo Fisher Scientific (CAT#: 9012-36-6)
14. ALBUMIN FROM BOVINE SERUM (MSDS):
    • Manufacturer: Sigma-Aldrich (CAT#: 9048-46-8)
15. ALLURA RED AC (MSDS):
    • Manufacturer: Santa Cruz Biotechnology (CAT#: sc-214523)
16. ALPHA-ACTIN:
    • Manufacturer: Thermo Fisher Scientific (CAT#: PIMA126928)
17. AMPICILLIN, SODIUM SALT (MSDS):
    • Manufacturer: Thermo Fisher Scientific (CAT#: 11593-027)
18. ARABINOSE (MSDS):
    • Manufacturer: Thermo Fisher Scientific (CAT#: 10323-20-3)
19. BORIC ACID (MSDS):
    • Manufacturer: Acros Organics (CAT#: 10043-35-3)
20. BSA STANDARD AMPULES (MSDS):
    • Manufacturer: Thermo Fisher Scientific (CAT#: 23209)
21. BROMPHENOL BLUE (MSDS):
    • Manufacturer: Thermo Fisher Scientific (CAT#: RDCB070010F)
22. CALCIUM CHLORIDE (MSDS):
    • Manufacturer: Sigma-Aldrich (CAT#: 10043-52-4)
23. CHELEX 100 RESIN (MSDS):
    • Manufacturer: Bio-Rad Laboratories (CAT#: 143-2832)
24. COOMASSIE BRILLIANT BLUE G-250 (MSDS):
    • Manufacturer: Thermo Fisher Scientific (CAT#: 6104-58-1)
25. COTTON TIPPED APPLICATORS:
    • Manufacturer: Puritan Medical Products Company LLC (REF#: 836-WC)
26. CITRIC ACID (MSDS):
    • Manufacturer: Sigma-Aldrich (CAT#: 77-92-9)
27. CUVETTES:
    • Manufacturer: Thermo Fisher Scientific (CAT#: 14955127)
28. DISODIUM PHOSPHATE (MSDS):
    • Manufacturer: EM Science (CAT#: 7558-79-4)
29. ECORI (MSDS):
    • Manufacturer: Thermo Fisher Scientific (CAT#: FD0275)
30. EDTA (MSDS):
    • Manufacturer: Thermo Fisher Scientific (CAT#: 02-002-786)
31. ETHANOL (MSDS):
    • Manufacturer: Sigma-Aldrich (CAT#: 64-17-5)
32. ETHIDIUM BROMIDE (MSDS):
    • Manufacturer: Thermo Fisher Scientific (CAT#: 15-585-011)
33. GELATIN (MSDS):
    • Manufacturer: Sigma-Aldrich (CAT#: 9000-70-8)
34. GOTAQ (MSDS):
    • Manufacturer: Thermo Fisher Scientific (CAT#: PRM3001)
35. GLYCEROL (MSDS):
    • Manufacturer: Thermo Fisher Scientific (CAT#: 17904)
36. GLYCINE (MSDS):
    • Manufacturer: Bio-Rad Laboratories (CAT#: 161-0718)
37. HINDIII (MSDS):
    • Manufacturer: Thermo Fisher Scientific (CAT#: FD0505)
38. HYDROCHLORIC ACID (MSDS):
    • Manufacturer: Thermo Fisher Scientific (CAT#: A142-212)
39. INOCULATING LOOPS 10QL SIZE:
    • Manufacturer: Copan Diagnostics Inc. (CAT#: CST-S10)
40. IODINE (MSDS):
    • Manufacturer: Thermo Fisher Scientific (CAT#: S186-1)
41. KANAMYCIN SULFATE (MSDS):
    • Manufacturer: Thermo Fisher Scientific (CAT#: 25389-94-0)
42. L-ARGININE (MSDS):
    • Manufacturer: Thermo Fisher Scientific (CAT#: BP2505100)
43. LAMBDA DNA (MSDS):
    • Manufacturer: Thermo Fisher Scientific (CAT#: FERSD0011)
44. LB AGAR (MSDS):
    • Manufacturer: Genesee Scientific (CAT#: 11-122)
45. LEPF1_T1 GENE PRIMER:
    • Manufacturer: Eurofins Scientific (CAT#: 1018836718)
46. LEPR1_T1 GENE PRIMER:
    • Manufacturer: Eurofins Scientific (CAT#: 1018836719)
47. M-13 ANTIBODY:
    • Manufacturer: Santa Cruz Biotechnology (CAT#: sc-47682)
48. MAGNESIUM CHLORIDE (MSDS):
    • Manufacturer: TaKaRa (CAT#: SD0315)
49. METHANOL (MSDS):
    • Manufacturer: Thermo Fisher Scientific (CAT#: 67-56-1)
50. METHYL GREEN (MSDS): 
    • Manufacturer: Thermo Fisher Scientific (CAT#: 7114-03-6)
51. METHYL ORANGE (MSDS):
    • Manufacturer: Sigma-Aldrich (CAT#: 547-58-0)
52. METHYLENE BLUE (MSDS):
    • Manufacturer: J.T. Baker (CAT#: 7220-79-3)
53. MICROSCOPE SLIDES (SINGLE FROSTED):
    • Manufacturer: Genesee Scientific (CAT#: 29-101)
54. MOLECULAR WEIGHT PROTEIN LADDER:
    • Manufacturer: Thermo Fisher Scientific (CAT#: PI84785)
55. MOPS (MSDS):
    • Manufacturer: Sigma Chemical Co. (CAT#: 214-478-5)
56. MUELLER HINTON LB BROTH (MSDS):
    • Manufacturer: Difco (CAT#: 275730)
57. NAPHTHOL (MSDS):
    • Manufacturer: Santa Cruz Biotechnology (CAT#: sc-213860)
58. NITROCELLULOSE MEMBRANE (MSDS):
    • Manufacturer: Whatman Inc. (CAT#: 10-402-452)
59. NP-40 (MSDS):
    • Manufacturer: Thermo Fisher Scientific (CAT#: 18-607-274)
60. NUCLEASE-FREE WATER:
    • Manufacturer: Thermo Fisher Scientific (CAT#: R0582)
61. ORANGE G (MSDS):
    • Manufacturer: EM Science (CAT#: 1936-15-8)
62. P-NITROPHENOL (MSDS):
    • Manufacturer: Thermo Fisher Scientific (CAT#: BP612-1)
63. PBS (PHOSPHATE BUFFERED SALINE) (MSDS):
    • Manufacturer: Thermo Fisher Scientific (CAT#: 70011044)
64. PENICILLIN G SODIUM SALT (MSDS):
    • Manufacturer: Thermo Fisher Scientific (CAT#: BP29555)
65. PEROXIDASE (MSDS):
    • Manufacturer: Thermo Fisher Scientific (CAT#: 89-956-436)
66. PETRI DISH:
    • Manufacturer: VWR Scientific (CAT#: 470210-568)
67. POTASSIUM ACETATE (MSDS):
    • Manufacturer: Sigma-Aldrich (CAT#: 127-08-2)
68. POTASSIUM CHLORIDE (MSDS):
    • Manufacturer: EM Science (CAT#: 7447-40-7)
69. POTASSIUM DIHYDROGEN PHOSPHATE (MSDS):
    • Manufacturer: Sigma-Aldrich (CAT#: 231-913-4)
70. PROTEINASE K (MSDS):
    • Manufacturer: Zymo Research (CAT#: D3001-2-B)
71. PSTI (MSDS):
    • Manufacturer: Thermo Fisher Scientific (CAT#: FD0615)
72. SAFRANIN O (MSDS):
    • Manufacturer: Thermo Fisher Scientific (CAT#: S670-25)
73. SDS (DODECYL SODIUM SULFATE) (MSDS):
    • Manufacturer: EM Science (CAT#: 151-21-3)
74. SODIUM BICARBONATE (MSDS):
    • Manufacturer: Mallinckrodt Chemical (CAT#: 144-55-8)
75. SODIUM BISULFITE (MSDS):
    • Manufacturer: Thermo Fisher Scientific (CAT#: 7631-90-5)
76. SODIUM CARBONATE (MSDS):
    • Manufacturer: EM Science (CAT#: 497-19-8)
77. SODIUM CHLORIDE (MSDS):
    • Manufacturer: Sigma-Aldrich (CAT#: 7647-14-5)
78. SODIUM HYDROXIDE (MSDS):
    • Manufacturer: Spectrum Chemical (CAT#: 1310-73-2)
79. STREPTOMYCIN SULFATE SALT (MSDS):
    • Manufacturer: Sigma-Aldrich (CAT#:3810-74-0)
80. STREPTAVIDIN-PEROXIDASE:
    • Manufacturer: Sigma-Aldrich (CAT#: 098K6001)
81. TAE BUFFER (MSDS):
    • Manufacturer: Thermo Fisher Scientific (CAT#: FERB49)
82. TMB (Tetramethylbenzidine) (MSDS):
    • Manufacturer: Thermo Fisher Scientific (CAT#: N301)
83. TRICINE (MSDS):
    • Manufacturer: Thermo Fisher Scientific (CAT#: 5704-04-1)
84. TRIS BASE (MSDS):
    • Manufacturer: Research Products International (CAT#: 77-86-1)
85. TRIS HYDROCHLORIDE (MSDS):
    • Manufacturer: J.T. Baker Inc. (CAT#: 1185-53-1)
86. TRITON X-100 (MSDS):
    • Manufacturer: Thermo Fisher Scientific (CAT#: 9002-93-1)
87. TWEEN 20 (MSDS):
    • Manufacturer: Research Products International (CAT#: 9005-65-6)
88. VF1_T1 GENE PRIMER:
    • Manufacturer: Eurofins Scientific (CAT#: 1018836720)
89. VR1_T1 GENE PRIMER:
    • Manufacturer: Eurofins Scientific (CAT#: 1018836721)
90. XYLENE CYANOL FF (MSDS):
    • Manufacturer: Sigma Chemical Co. (CAT#: 2650-17-1)