Biotechnology Laboratory Activities

In addition to all of the laboratory activities and support provided by the UA BIOTECH Project, this partnership is working on developing new laboratory activities:

• Penicillium Antibiotic Effect
  • [Student Guide] [Materials Guide]

 

• SDS-Polyacrylamide Gel Electrophoresis (No Protein Concentration assay)

  • [Student Guide] [Materials Guide]

 

• SDS-Polyacrylamide Gel Electrophoresis with Bradford Assay

  • [Student Guide] [Materials Guide]

 

• Western Blotting

  • [Student Guide] [Materials Guide]

 

• Enzymatic Activity of Cellobiase: (aka BioFuels from BioRad)

  • One ml cuvette version

    • [Student Guide] [Materials Guide]

  •  Four ml cuvette version

    • [Student Guide] [Materials Guide]

 

• PCR for 35S Promoter in Corn

  • [Student Guide] [Materials Guide]

 

• Determine If Your Food is Genetically Modified

  • [Student Guide] [Materials Guide]

  • Extra Reading:

    • The New Breed
    • A Hard Look at GM Crops

 

• DNA Barcoding Sequence Analysis using DNA Subway

  • [Student Guide] [Materials Guide]

  • Extra reading:

    • Scientific American

    • Stoeckle

    • Herbert

 

• Mitochondrial DNA--Gateway to Your Heredity

  • [Student Guide] [Materials Guide]

 

• Comparing PCR of a Single Loci to Restriction Digest of the Entire Genome

  • [Student Guide] [Materials Guide] 

 

• Identification of Bacterial Species
  • [Student Guide] [Materials Guide]
  • Extra Material:
    • Antibiotic Sensitivity Testing
    • Gram Staining
  • Extra Reading:
    • 16S rDNA Sequence Analysis

 

• Transformation with GFP

 

• PCR of GFP (staining with Methylene Blue)

  • [Student Guide] [Materials Guide]

 

• PCR of GFP (staining with Ethidium Bromide)

  • [Student Guide] [Materials Guide]

  • Extra Material:

    • Sequencing the PCR Product

    • GFP sequence

    • Regulation of pBAD promoter

 

• Design Your Primer

  • [Student Guide] [Materials Guide]

 

• C. elegans Mutant Genetic

  • [Student Guide] [Materials Guide]

  • Extra material:

    • Nemotode Growth Media and Passaging of C. elegan

    • C. elegan Life Cycle:

      Image