Virtual Transformation/PCR/BLAST Activity


Virtual Instruction of Bacterial Transformation with PCR and BLAST - These lessons were designed by Rick Heupel from Salpointe High School to be able to utilize BIOTECH Project Transformation and PCR lab activities for his biotechnology students

Introduction Lesson

Lesson 1-Transformation

    Bacterial transformation GFPplasmid

Lesson 2-Transformation/PCR

    PCR explained 8 minute video by miniPCR (product advertisement at the end)

    PCR_GFP in pBAD-GFPuv

Lesson 3-BLAST

   Determine sequence of PCR

   Sequence Results

Lesson 4-Regulation Hypothesis

    pBAD-GFPuv regulated expression

Lesson Summary